Antimicrobial Performance Evaluation of Textiles using a Absorption Method

The study aims to evaluate the antimicrobial performance of textiles using an absorption method. The textile samples were exposed to a microbial solution containing Escherichia coli and Staphylococcus aureus bacteria. The samples were then evaluated for their ability to absorb the bacteria over a period of 24 hours. The results showed that the textiles with higher fiber length had better antibacterial properties than those with shorter fibers. Additionally, the use of natural fibers such as cotton and bamboo had significantly better antimicrobial properties compared to synthetic fibers. The study highlights the importance of choosing appropriate textiles with high-quality fibers in preventing the growth and spread of harmful microorganisms on fabrics. This method provides a useful tool for evaluating the effectiveness of textiles in controlling bacterial contamination in healthcare settings, food production, and other applications where微生物 control is critical.

Abstract: The global demand for antibacterial and antiviral technologies in textiles has increased exponentially due to the emergence of infectious diseases. To meet this demand, there is a growing interest in developing effective and efficient methods for assessing the antibacterial effectiveness of textiles. In this study, we propose a new method for assessing the antibacterial performance of textiles using an absorption method. This method involves testing the ability of textiles to absorb and remove bacteria from aqueous solutions. We evaluate the performance of different textile materials and demonstrate that our method provides accurate and reliable results. Our findings have significant implications for the development of textile products with enhanced antibacterial properties.

Introduction:

Antimicrobial infections are one of the leading causes of death worldwide, with millions of deaths attributed to bacterial and viral infections every year. The World Health Organization (WHO) estimates that over 500,000 deaths occur due to infection-related illnesses each year. The rise in antibiotic resistance has made it increasingly difficult to treat infections effectively. Therefore, there is a need for new technologies that can provide effective protection against bacterial and viral infections. One such technology is antimicrobial agents integrated into textiles. However, it is challenging to develop textiles that are both durable and resistant to microbial growth. To address this challenge, there is a need for reliable methods that can assess the antibacterial efficacy of textiles.

Method:

In this study, we developed an absorption method for assessing the antibacterial performance of textiles. The method involves testing the ability of textiles to absorb and remove bacteria from aqueous solutions. The test involves placing the textile samples in a solution containing a known number of bacterial cells. The samples are then incubated at various temperatures for a specified period. Following incubation, the number of remaining bacterial cells in the solution is measured. The amount of bacteria absorbed by the textile samples is compared to the number of bacteria present in the solution at the beginning of the incubation period. The results are used to calculate the antibacterial effectiveness of the textile samples.

Materials and Methods:

1. Textile Sample Preparation: A total of ten different textile materials were tested, including cotton, polyester, rayon, and nylon. Each sample was cut into squares of equal size (25 x 25 cm).

2. Solution Preparation: A solution containing Escherichia coli (E. coli) bacteria was prepared using a stock solution containing 10^6 E. coli cells/mL in PBS (phosphate-buffered saline) with 0.1% Tween-80 (Tween-80 is used to reduce non-reactive background). The solution was adjusted to have a final concentration of 10^4 E. coli cells/mL.

3. Incubation Process: The textile samples were placed in separate wells in a 96-well plate and incubated at three different temperatures: 20°C, 40°C, and 60°C. Each temperature was tested for two hours. After incubation, the supernatant was aspirated and filtered using a microfiltration membrane (MilliporeTM Filter Unit 10K). The filtrated supernatant was then analyzed using an automated reader (Bio-Rad Model XRS).

4. Data Analysis: The data were analyzed using GraphPad Prism software v8.07 (GraphPad Software Ltd). The absorbance values were normalized using the average absorbance values of control solutions (pH = 7.4) containing no bacteria or E. coli cells. The results were then compared between different textile materials and temperatures using one-way ANOVA analysis. The P-values were calculated using a posthoc test (Tukey's HSD test).

Results:

The results showed that all textile samples had some degree of antibacterial activity when tested under different temperatures. Cotton samples had the lowest level of antibacterial activity, while nylon samples had the highest level of activity. At 20°C, only a few E. coli cells could be detected in the supernatant after incubation for two hours. However, at 40°C and 60°C, most of the E. coli cells were removed from the supernatant. The levels of antibacterial activity increased with increasing temperature, indicating that higher temperatures promote faster removal of bacteria from textile materials. Furthermore, we observed that polyester fibers had higher levels of antibacterial activity than rayon fibers when tested under different temperatures. This result suggests that polyester fibers may be more suitable for developing textiles with enhanced antibacterial properties.

Conclusion:

In conclusion, our study demonstrates that an absorption method can be used to assess the antibacterial performance of textiles. Our results show that different textile materials have varying levels of antibacterial activity when tested under different temperatures. Polyester fibers appear to have higher levels of antibacterial activity than rayon fibers when tested under high temperatures

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